FracLac v 1.2 for ImageJ

FracLac v 1.2 for ImageJ

 

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·  Installation

·  Overview

·  FracLac Options

 

FracLac2004 is a plug-in for ImageJ (biological image analysis software written and made available to the scientific community by Wayne Rasband of the NIH).

 

Use FracLac to automatically analyse morphological complexity of single or multiple images or parts of images (e.g., different cells in one image). FracLac is suitable for images of biological structures (e.g., neurons or microglia, cell nuclei), theoretical fractals, textures, and more.

Author: Audrey Karperien

Charles Sturt University

email: akarpe01@postoffice.csu.edu.au

FracLac was originally developed by Audrey Karperien, CSU, based on algorithm's by Herbert Jelinek, PhD, Neuroscience, School of Community Health, Charles Sturt University with guidance from Wayne Rasband (NIH). The convex hull algorithm was originally contributed by Thomas R. Roy, University of Alberta, Canada.

JavaDoc and Development History

Installation instructions: Download the Frac_Lac.jar file into the ImageJ plugins folder, restart ImageJ, then run FracLac. A shortcut appears in the Plugins>analyze menu.

 

Download the most recent beta FracLac v 1.2 .

The download for this ongoing project build is compiled on java 1.4.2_04 but includes source files in the jar archive as well. A version compiled on 1.5.0 is available (download for 1.5.0 only); it does not run on ImageJ using 1.4.2_04. The previous release is available from the ImageJ plugins site.

How to use it:

  • Simply put the downloaded .jar file in the ImageJ plugins directory.
  • Start ImageJ, select the Plug-ins>Analyze menu, and click on FracLac_(current version).
  • Before analyzing an image or roi, you must select from the popup the type of analysis you want. Clicking on the type you want opens a panel for setting options (choose OK for the default settings).
  • Once a type of analysis is chosen (the message at the bottom of the panel tells what type is selected), analyze the currently active image or roi by clicking the DO CURRENT IMAGE button.
  • To analyze multiple files, click on the DO NEW FILES button. A file opening dialog will appear, from which several files can be selected at once (shift and select or control and select). When the files are highlighted, click OPEN on the dialog and they will be analyzed using the most recent settings.

Results:

FracLac delivers the results in a text file and graphically if you choose to display graphs. To continue analysing images, use the buttons on the
FracLac Panel. For detailed instructions, read on.

 

 

 

FracLac delivers:

 

copyright (c) 2002-4 Audrey Karperien

Contact the author to download the most recent beta version of a java standalone application that calculates the multifractal spectrum based on the probability distribution; the data are extensive and clutter the results file in ImageJ.